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gmk [2019-09-26 08:54:56]
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gmk [2019-09-26 08:54:56]

guanylate kinase (GMP:dATP, dGMP:ATP)
locus
BSU_15680
pI
4.62
mw
23.07 kDa
protein length
204 aa Sequence Blast
gene length
615 bp Sequence Blast
function
GTP biosynthesis
product
guanylate kinase (GMP:dATP, dGMP:ATP)
essential
yes
ec
2.7.4.8
synonyms
yloD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    1,641,949 1,642,563

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + GMP --> ADP + GDP (according to UniProt)

    • Protein family

  • guanylate kinase family (single member, according to UniProt)

    • [SW|Domains]

  • Guanylate kinase-like domain (aa 5-184) (according to UniProt)

    • Modification

  • phosphorylated on Arg-134 and/or Arg-149[Pubmed|22517742]

    • Effectors of protein activity

  • inhibition of enzymatic activity by (p)ppGpp during the stringent response[Pubmed|22981860]

    • Structure

  • [PDB|1S4Q] (from ''Mycobacterium tuberculosis h37rv'', 39% identity, 60% similarity)
  • [http://pdb.org/pdb/search/structidSearch.do?structureId=4QRH 4QRH] (from ''Staphylococcus aureus'', complex with pppGpp) [Pubmed|25661490]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

    description

  • [pubmed|22383849]

    • sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|23396918], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulation

  • expressed during vegetative growth and early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|23396918]
    • view in new tab

    genes
    [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

    description

  • [pubmed|22383849]
    • view in new tab

    genes
    [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

    description

  • [pubmed|22383849]

    • regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]
    • view in new tab

    References

    Reviews

  • 27149325

    • Original publications

  • 22517742,22981860,24722991,24682323,25661490